Camelina sativa variety “SO-110”

ABSTRACT

The subject invention relates to a Camelina sativa (L.) Crantz spring-type seed designated as “SO-110” derived from a cross between camelina accessions with high yield and oil quality attributes following conventional breeding methodologies.

CROSS-REFERENCE TO RELATED APPLICATION

This application claims priority to U.S. Provisional Patent App. No.63/120,285, filed Dec. 2, 2020, which is incorporated herein byreference in its entirety.

BACKGROUND

Current trends in the international petroleum market and concerns on theexcessive use of petroleum-derived fuels on the environment have led toincreased interest in the development and adoption of renewable sourcesof energy in the USA. In some instances this has derived in the adoptionof government policies, like the Energy Independency and Security Act of2007 (Public Law 110-140, 2007), in others in the take-over of privateinitiatives like that aimed at using plant-derived renewable fuels topartly satisfy the fuel demand of the aviation industry (Anonymous,2009).

Among the several types of feedstocks proposed for the production ofrenewable fuel, use of industrial-grade oilseed crops are considered aviable option. Camelina (Camelina sativa, (L.) Crantz), an annual plantthat belongs to the Brassicaceae family, is an oilseed crop that canproduce decent yields under relative low inputs, exhibits a broadadaptability to a range of environmental conditions, and its seedscontain a relatively high amount of oil (Putnam et al., 1993; Budin etal., 1995; Vollman et al., 1996; Gugel and Falk, 2006). In addition,studies on the impact of camelina-derived fuel on the environmentindicates that use of this fuel can reduce carbon emissions by up to 80%(Shonnard et al., 2010) conferring this crop a potential to be used asbiofuel feedstock crop.

Although camelina is a plant with a rich history (Schultze-Motel, J.,1979; Bouby, 1998), in general little genetic improvement has beenpracticed on this crop. In the USA, although efforts were devoted tothis crop in the past (Porcher, 1863, Robinson, 1987), currently thenumber of varieties available for commercial production is very limited.Consequently, there is a real need to develop camelina varieties withhigh productivity and broad adaptability, especially to low-inputagricultural systems in the USA, to be used as reliable, commercialfeedstocks for the emerging biofuel industry.

The main object of the invention is to provide seed of a superiorcamelina variety that provides high and stable yields and is suitable ofcommercial production under low-input and/or high-input conditions inagricultural areas in various temperate regions. Another object is toprovide seed of a camelina variety that exhibits acceptable and stableagronomic characteristics.

BRIEF SUMMARY OF THE INVENTION

The subject invention provides camelina plants having uniquereproductive attributes associated with grain and oil yield superiority,and the ability to grow efficiently and consistently under dryland,low-input and/or high-input conditions. The subject invention alsoprovides methods of producing the camelina plants, as well as methods ofintroducing one or more desired traits into the camelina plants.

In preferred embodiments, the camelina plants of the subject inventionare of the Camelina sativa (L.) variety. In specific preferredembodiments, the Camelina sativa (L.) variety is the camelina plantdesignated as “SO-110,” a representative seed sample of which has beendeposited under ATCC Accession No. PTA-126939 on Dec. 18, 2020.

In some embodiments, the camelina plant is a plant having one or more,or all the physiological and morphological characteristics of Camelinasativa (L.) variety “SO-110.” In some embodiments, the camelina plant isderived from a cross between a first parent camelina plant and a secondparent camelina plant, wherein the first and/or second camelina plantsare Camelina sativa (L.) variety “SO-110,” or camelina plants having oneor more, or all, of the physiological and morphological characteristicsof Camelina sativa (L.) variety “SO-110.”

In preferred embodiments, the camelina plants of the subject inventionhave high grain and oil yields compared to a check line of camelina. Insome embodiments, the check line is, for example, Calena, Ligena, BlaineCreek, Cs11, Cs21, Celine, Galena, Robinson, and/or Suneson.

In certain embodiments, the camelina plants of the subject inventionhave higher grain and oil yields when compared with Camelina sativa (L.)variety “SO-50,” a description of which can be found in U.S. Pat. No.8,319,021 (incorporated by reference herein).

In some embodiments, the camelina plants of the subject invention aredeveloped through conventional breeding methods, having the ability togrow efficiently and consistently under dryland, low-input and/orhigh-input conditions.

In some embodiments, the distinctive features of the subject plantscompared to a check line include one or more of: a prolonged seedfilling period (e.g., about 33 days), an increased seed weight (e.g.,about 1.38 g/1,000), a decreased test weight (e.g., about 50.0 Lb/Bu),an increased seed oil content (e.g., about 36.2%), a higher grain yield(e.g., about 1,582 lb/ac) and a higher oil yield (e.g., about 573lb/ac). Additionally, the plants are stable in their performance acrossa wide range of environmental conditions (see FIGS. 1-2 ).

In certain embodiments, the subject invention also provides plant partsof the subject camelina plants. Plant parts can include, for example,the shoot, root, stem, seeds, racemes, stipules, leaves, petals,flowers, ovules, bracts, branches, petioles, internodes, pollen, stamen,or the like.

In some embodiments, the plant part is the seed of the camelina plantdesignated as “SO-110” (ATCC No. PTA-126939).

In certain embodiments, the subject invention also provides plant cellsof the subject camelina plants. In some embodiments, the plant cell(s)can be cultured and used to produce a camelina plant having one or more,or all, of the physiological and morphological characteristics of thesubject camelina plants.

In certain embodiments, the subject invention also provides tissueculture of the subject camelina plants. In some embodiments, the tissueculture(s) are produced from a plant part such as, for example, embryos,meristematic cells, leaves, pollen, root, root tips, stems, anther,pistils, pods, flowers, and seeds. In some embodiments, the tissueculture can be used to regenerate a Camelina sativa (L.) plant, saidplant having the morphological and physiological characteristics ofCamelina sativa (L.) variety “SO-110” (ATCC No. PTA-126939).

In certain embodiments, the subject invention provides methods ofproducing the subject camelina plants. In some embodiments, the plantsare produced through conventional breeding methods.

In certain embodiments, the subject invention provides methods forproducing a camelina seed. In some embodiments, the methods comprisecrossing a first parent camelina plant with a second parent camelinaplant and harvesting the resultant hybrid seed, wherein the first parentcamelina plant and/or second parent camelina plant is a Camelina sativa(L.) plant of the subject invention.

In certain embodiments, the subject invention provides methods forintroducing one or more desired traits into the subject camelina plants.

In some embodiments, the methods comprise introducing one or moretransgenes into the genome of the subject camelina plants. In someembodiments, the methods comprise crossing the camelina plants of thesubject invention to one or more transgenic plants, wherein thetransgenic plants comprise one or more transgenes.

In some embodiments, the transgene is a gene for herbicide resistance ina plant, and the herbicide is selected from the group consisting ofimidazolinone, sulfonylurea, glyphosate, glufosinate,L-phosphinothricin, triazine, sethoxydim, and benzonitrile. In someembodiments, the transgene is a gene for insect resistance in a plant,for example, the transgene encodes a Bacillus thuringiensis endotoxin.In some embodiments, the transgene is a gene for disease resistant in aplant. In some embodiments, the transgene is a gene for water stresstolerance, heat tolerance, improved shelf life, and/or improvednutritional quality.

In some embodiments, the methods for introducing one or more desiredtraits into the subject camelina plants comprise:

(a) crossing a camelina plant of the subject invention with anothercamelina plant that comprises a desired trait to produce F1 progenyplants;

(b) selecting one or more progeny plants that have the desired trait toproduce selected progeny plants;

(c) crossing the selected progeny plants with the camelina plant of thesubject invention to produce backcross progeny plants;

(d) selecting for backcross progeny plants that have the desired traitand physiological and morphological characteristics of the camelinaplant of the subject invention to produce selected backcross progenyplants; and

(e) optionally, repeating steps (c) and (d) three or more times insuccession to produce selected fourth or higher backcross progeny plantsthat comprise the desired trait and the physiological and morphologicalcharacteristics of the camelina plant of the subject invention.

In some embodiments, the desired trait is, for example, selected fromthe group consisting of insect resistance, disease resistance, waterstress tolerance, heat tolerance, improved shelf life, and improvednutritional quality.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 shows a stability of performance curve for SO-110, Calena andBlaine Creek varieties.

FIG. 2 shows the linear regression coefficient and residual variancefrom stability of performance analyses of SO-110, Blaine Creek andCalena according to Finlay and Wilkinson (1963) and Eberhart and Russell(1966).

DETAILED DESCRIPTION OF THE INVENTION

The subject invention provides camelina plants having reproductiveattributes that lead to high grain yield and high oil yield, and theability to grow efficiently and consistently under dryland, low-inputand/or high-input conditions. The subject invention also providesmethods of producing the camelina plants, as well as methods ofintroducing one or more desired traits into the camelina plants.

Selected Definitions

As used herein:

“Days to 50% flowering” refers to period from germination of the seed tothe manifestation of flowering in 50% of the plant population;

“Days to Maturity” refers to the period from germination of the seed tothe period when fully developed seeds where developed in 50% of theplant population;

“Seed filling days” refers to the period from the beginning of seedgrowth until the seed is fully developed and has reached maximum dryweight;

“Plant height” refers to the height of the adult plant from the groundbase where it is being grown to the tip of the main raceme;

“Racemes per plant” refers to the number of reproductive branchesderived from the main stem of the plant;

“Main raceme length” refers to the length of the terminal raceme in theplant;

“Inflorescence length” refers to the length of the main inflorescencefrom its base to the tip of the terminal raceme;

“Inflorescence diameter” refers to the diameter of the inflorescence atits widest plane and is measured right after flowering has beencompleted;

“Pod number” refers to the total number of pods in the plant bearingseeds;

“Pod weight” refers to the weight of a pod once the plant has reachedmaturity and consequently is ready to be harvested;

“Seeds per pod” refers to the number of fully developed seed containedinside a pod in the plant;

“Seeds per plant” refers to the total number of fully developed seedsthe plant has produced;

“Seed weight” refers to the total weight of a fully developed seed,usually expressed in weight per thousand seeds;

“Test weight” refers to a measure of the seed weight in pounds for agiven bushel volume;

“Grain yield” refers to a measure of the harvested clean seed weight inpounds in one acre of land area;

“Oil content” refers to the fraction of total oil contained in themature seed;

“Oil yield” refers to a measure of the seed oil weight collected inpounds in one acre of land area;

“Variety” refers to a homogeneous, highly homozygous group ofindividuals that are genetically distinct from other groups ofindividuals within the species;

“Cross” refers to the process by which pollen from one flower from aplant is artificially transferred to the stigma from the flower ofanother plant;

“Progeny” refers to the offspring derived from an artificial crossbetween two plants;

“Selfing” refers to the manifestation of the process ofself-pollination, which in turn refers to the transfer of pollen fromthe anther of a flower to the stigma of the same flower or differentflowers on the same plant;

“Single plant selection” refers to a form of selection in which plantswith specific desirable attributes are identified and individuallyselected; and

“Seed increase” refers to the process of sowing, growing and harvestingseed from a specific plant material for the purpose of creating a largervolume of seed.

As used herein, the term “plant” refers to any living organism belongingto the kingdom Plantae (i.e., any genus/species in the Plant Kingdom).In preferred embodiments, the plant is a species in the tribe ofCamelineae, such as Camelina alyssum, C. anomala, C. grandiflora, C.hispida, C. laxa, C. microcarpa, C. microphylla, C. persistens, C.rumelica, C. sativa, C. stiefelhagenii, and/or any hybrid thereof.

As used herein, the terms “plant part” and “plant tissue” are usedinterchangeably and refer to any portion(s) of a plant including, butnot limited to, the shoot, root, stem, seeds, racemes, stipules, stalks,leaves, petals flowers, ovules, bracts, branches, petioles, internodes,tiller, pollen, stamen, and the like. “Aerial” plant parts refer toabove-ground plant parts, which can also be referred to as “shoots.”“Subterranean” plant parts refer to below-ground plant parts, which canalso be referred to as “roots.”

As used herein, the term “cross,” “crossing,” “cross-pollination” or“cross-breeding” refer to the process by which the pollen of one floweron one plant is applied (artificially or naturally) to the ovule(stigma) of a flower on another plant.

As used herein, the term “gene” refers to any segment of DNA associatedwith a biological function. Thus, genes include, but are not limited to,coding sequences and/or the regulatory sequences required for theirexpression. Genes can also include non-expressed DNA segments that, forexample, form recognition sequences for other proteins. Genes can beobtained from a variety of sources, including cloning from a source ofinterest or synthesizing from known or predicted sequence information,and may include sequences designed to have desired parameters.

The transitional term “comprising,” which is synonymous with“including,” or “containing,” is inclusive or open-ended and does notexclude additional, unrecited elements or method steps. By contrast, thetransitional phrase “consisting of” excludes any element, step, oringredient not specified in the claim. The transitional phrase“consisting essentially of” limits the scope of a claim to the specifiedmaterials or steps “and those that do not materially affect the basicand novel characteristic(s)” of the claimed invention. Use of the term“comprising” contemplates other embodiments that “consist” or “consistessentially” of the recited component(s).

Unless specifically stated or obvious from context, as used herein, theterm “or” is understood to be inclusive. Unless specifically stated orobvious from context, as used herein, the terms “a,” “and” and “the” areunderstood to be singular or plural.

Unless specifically stated or obvious from context, as used herein, theterm “about” is understood as within a range of normal tolerance in theart, for example within 2 standard deviations of the mean. About can beunderstood as within 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5%,0.1%, 0.05%, or 0.01% of the stated value.

The recitation of a listing of chemical groups in any definition of avariable herein includes definitions of that variable as any singlegroup or combination of listed groups. The recitation of an embodimentfor a variable or aspect herein includes that embodiment as any singleembodiment or in combination with any other embodiments or portionsthereof.

All references cited herein are hereby incorporated by reference intheir entirety.

Camelina sativa

Camelina sativa, known in English as camelina, gold-of-pleasure, falseflax, wild flax, linseed dodder, German sesame, and Siberian oilseed, isa flowering plant in the family Brassicaceae, which includes, forexample, mustard, cabbage, rapeseed, broccoli, cauliflower, kale, andbrussels sprouts. Camelina is native to Northern Europe and to CentralAsia, but has been introduced to North America, possibly as a weed inflax imports.

The crop is now being researched due to its exceptionally high levels(up to 45%) of omega-3 fatty acids, which is uncommon in vegetablesources. The fatty acid composition of camelina comprises high levels ofpolyunsaturated fatty acids, such as C18:2 and C18:3 fatty acids(52-54%), as well as long chain fatty acids, such as C20:1 (11-15%) andC22:1 (2-5%) fatty acids.

Camelina oil is well-suited for use as a cooking oil. It has analmond-like flavor and aroma. The major components of camelina oil arealpha-linolenic acid (C18:3 (omega-3-fatty acid, approximately 35-45%)and linoleic acid (C18:2 (omega-6 fatty acid, approximately 15-20%)).Camelina oil is also very rich in natural antioxidants, such astocopherols, which contributes to the highly stable character of theoil, as well as its resistance to oxidation and rancidity. Othercomponents of camelina oil include, for example, erucic acid (1-3%) andvitamin E (about 110 mg/100 g). (Pilgeram et al., 2007; Vollmann et al.1996; Putnam et al., 1993; Berti and Schneiter, 1993; Pavlista andBaltensperger, 2007).

In addition to its oil, camelina plant parts can be used as commercialfeed, as well as to produce useful chemicals, for example, camalexins(Browne et al., 1991).

Methods of transforming camelina plants have been described inUS20040031076, US20090151028, US20090151023, WO/2002/038779A1, andWO/2009/117555A1, each of which is incorporated by reference in itsentirety.

Methods for camelina tissue culture have been described previously. Forexample, Camelina sativa shoots have been regenerated from leaf explants(Tattersall and Millam, Plant Cell Tissue and Organ Culture 55:147-149,1999). Camelina sativa has also been used in a somatic fusion with otherBrassica species (Narasimhulu et al., Plant Cell Rep. 13:657-660, 1994;Hansen, Crucifer. News 19:55-56, 1997; Sigareva and Earle, Theor. Appl.Genet. 98:164-170, 1999) and regenerated interspecific hybrid plantswere obtained (Sigareva and Earle).

More tissue culture techniques for Camelina can be found in Bhojwani andRazdan (Plant tissue culture: theory and practice, Elsevier, 1996, ISBN97804448162328), Trigiano and Gray (Plant tissue culture concepts andlaboratory exercises, Volume 1999, CRC Press, 2000, ISBN 0849320291,9780849320293), Kumar (Plant Tissue Culture And Molecular Markers: TheirRole In Improving Crop Productivity, I. K. International Pvt Ltd, 2009,ISBN 8189866109, 9788189866105), George et al., (Plant Propagation byTissue Culture 3rd Edition: Volume 1. the Background, ISBN 1402050046,9781402050046). Sathyanarayana (Plant Tissue Culture: Practices and NewExperimental Protocols, I. K. International Pvt Ltd, 2007, ISBN8189866117, 9788189866112), Pierik (In vitro culture of higher plants,Springer, 1997, ISBN 0792345274, 9780792345275), and Vasil (Plant celland tissue culture, Springer, 1994, ISBN 0792324935, 9780792324935),each of which is incorporated by reference in its entirety herein forall purposes.

Camelina sativa (L.) Variety “SO-110”

Camelina sativa (L.) variety “SO-110” is a true-bred camelina selectedfrom a cross between accession “Ames1043,” a material originated inPoland, and accession “PI 304268,” a material originated in Sweden. Arepresentative sample of seeds of “SO-110” has been deposited under ATCCAccession No. PTA-126939 on Dec. 18, 2020.

Breeding Methods

Open-Pollinated Populations. The improvement of open-pollinatedpopulations of such crops as rye, many maizes and sugar beets, herbagegrasses, legumes such as alfalfa and clover, and tropical tree cropssuch as cacao, coconuts, oil palm and some rubber, depends essentiallyupon changing gene-frequencies towards fixation of favorable alleleswhile maintaining a high (but far from maximal) degree ofheterozygosity. Uniformity in such populations is impossible andtrueness-to-type in an open-pollinated variety is a statistical featureof the population as a whole, not a characteristic of individual plants.Thus, the heterogeneity of open-pollinated populations contrasts withthe homogeneity (or virtually so) of inbred lines, clones and hybrids.

Population improvement methods fall naturally into two groups, thosebased on purely phenotypic selection, normally called mass selection,and those based on selection with progeny testing. Interpopulationimprovement utilizes the concept of open breeding populations; allowinggenes to flow from one population to another. Plants in one population(cultivar, strain, ecotype, or any germplasm source) are crossed eithernaturally (e.g., by wind) or by hand or by bees (commonly Apis melliferaL. or Megachile rotundata F.) with plants from other populations.Selection is applied to improve one (or sometimes both) population(s) byisolating plants with desirable traits from both sources.

There are several primary methods of open-pollinated populationimprovement. First, there is the situation in which a population ischanged en masse by a chosen selection procedure. The outcome is animproved population that is indefinitely propagable by random-matingwithin itself in isolation. Second, the synthetic variety attains thesame end result as population improvement but is not itself propagableas such; it has to be reconstructed from parental lines or clones.Third, a method used in plant species that are largely self-pollinatedin nature, such as soybeans, wheat, rice, safflower, camelina and othersis pedigree selection. In this situation, crosses are made andindividual plants and lines from individual plants are selected fordesired traits. These lines are then advanced as genetically homogeneousvarieties. Since the individuals are largely self-pollinated these linesare analogous to an inbred line with favorable agronomiccharacteristics. These plant breeding procedures for improvingopen-pollinated populations are well known to those skilled in the artand comprehensive reviews of breeding procedures routinely used forimproving cross-pollinated plants are provided in numerous texts andarticles, including: Allard, Principles of Plant Breeding, John Wiley &Sons, Inc. (1960); Simmonds, Principles of Crop Improvement, LongmanGroup Limited (1979); Hallauer and Miranda, Quantitative Genetics inMaize Breeding, Iowa State University Press (1981); and, Jensen, PlantBreeding Methodology, John Wiley & Sons, Inc. (1988).

Mass Selection. In mass selection, desirable individual plants arechosen, harvested, and the seed composited without progeny testing toproduce the following generation. Since selection is based on thematernal parent only, and there is no control over pollination, massselection amounts to a form of random mating with selection. As statedabove, the purpose of mass selection is to increase the proportion ofsuperior genotypes in the population.

Synthetics. A synthetic variety is produced by crossing inter se anumber of genotypes selected for good combining ability in all possiblehybrid combinations, with subsequent maintenance of the variety by openpollination. Whether parents are (more or less inbred) seed-propagatedlines, as in some sugar beet and beans (Vicia) or clones, as in herbagegrasses, clovers and alfalfa, makes no difference in principle. Parentsare selected on general combining ability, sometimes by test crosses ortopcrosses, more generally by polycrosses. Parental seed lines may bedeliberately inbred (e.g. by selfing or sib crossing). However, even ifthe parents are not deliberately inbred, selection within lines duringline maintenance will ensure that some inbreeding occurs. Clonal parentswill, of course, remain unchanged and highly heterozygous.

Whether a synthetic can go straight from the parental seed productionplot to the farmer or must first undergo one or two cycles ofmultiplication depends on seed production and the scale of demand forseed. In practice, grasses and clovers are generally multiplied once ortwice and are thus considerably removed from the original synthetic.

While mass selection is sometimes used, progeny testing is generallypreferred for polycrosses, because of their operational simplicity andobvious relevance to the objective, namely exploitation of generalcombining ability in a synthetic.

The number of parental lines or clones that enter a synthetic varywidely. In practice, numbers of parental lines range from 10 to severalhundred, with 100-200 being the average. Broad based synthetics formedfrom 100 or more clones would be expected to be more stable during seedmultiplication than narrow based synthetics.

Pedigreed varieties. A pedigreed variety is a superior genotypedeveloped from selection of individual plants out of a segregatingpopulation followed by propagation and seed increase of self-pollinatedoffspring and careful testing of the genotype over several generations.This is an open pollinated method that works well with naturallyself-pollinating species. This method can be used in combination withmass selection in variety development. Variations in pedigree and massselection in combination are the most common methods for generatingvarieties in self-pollinated crops.

Hybrids. A hybrid is an individual plant resulting from a cross betweenparents of differing genotypes. Commercial hybrids are now usedextensively in many crops, including corn (maize), sorghum, sugarbeet,sunflower and broccoli. Hybrids can be formed in a number of differentways, including by crossing two parents directly (single cross hybrids),by crossing a single cross hybrid with another parent (three-way ortriple cross hybrids), or by crossing two different hybrids (four-way ordouble cross hybrids).

Strictly speaking, most individuals in an out breeding (i.e.,open-pollinated) population are hybrids, but the term is usuallyreserved for cases in which the parents are individuals whose genomesare sufficiently distinct for them to be recognized as different speciesor subspecies. Hybrids may be fertile or sterile depending onqualitative and/or quantitative differences in the genomes of the twoparents. Heterosis, or hybrid vigor, is usually associated withincreased heterozygosity that results in increased vigor of growth,survival, and fertility of hybrids as compared with the parental linesthat were used to form the hybrid. Maximum heterosis is usually achievedby crossing two genetically different, highly inbred lines.

The production of hybrids is a well-developed industry, involving theisolated production of both the parental lines and the hybrids whichresult from crossing those lines. For a detailed discussion of thehybrid production process, see, e.g., Wright, Commercial Hybrid SeedProduction 8:161-176, In Hybridization of Crop Plants.

Additional breeding methods have been known to one of ordinary skill inthe art, e.g., methods discussed in Chahal and Gosal (Principles andprocedures of plant breeding: biotechnological and conventionalapproaches, CRC Press, 2002, ISBN 084931321X, 9780849313219), Taji etal. (In vitro plant breeding, Routledge, 2002, ISBN 156022908X,9781560229087), Richards (Plant breeding systems, Taylor & Francis US,1997, ISBN 0412574500, 9780412574504), Hayes (Methods of Plant Breeding,READ BOOKS, 2007, ISBN 1406737062, 9781406737066), and LOrz et al.(Molecular marker systems in plant breeding and crop improvement,Springer, 2005, ISBN 3540206892, 9783540206897), each of which isincorporated by reference in its entirety.

Deposit Information

A deposit of the seed of Camelina sativa (L.) variety “SO-110” ismaintained by Sustainable Oils, Inc., 2790 Skypark Dr. Suite 105,Torrance, Calif. 90505, USA. In addition, a sample of the seed ofCamelina sativa (L.) variety “SO-110” has been deposited by SustainableOils, Inc. with American Type Culture Collection (ATCC), 10801University Blvd. Manassas, Va. 20110-2209, USA.

To satisfy the enablement requirements of 35 U.S.C. § 112, and tocertify that the deposit of the seeds of the present invention meets thecriteria set forth in 37 C.F.R. §§ 1.801-1.809, Applicants hereby makethe following statements regarding the deposited seed of Camelina sativa(L.) variety “SO-110” (deposited as ATCC Accession No. PTA-126939 onDec. 18, 2020):

-   -   1. During the pendency of this application, access to the        invention will be afforded to the Commissioner upon request;    -   2. Upon granting of the patent the strain will be available to        the public under conditions specified in 37 CFR § 1.808;    -   3. The deposit will be maintained in a public repository for a        period of 30 years or 5 years after the last request or for the        enforceable life of the patent, whichever is longer;    -   4. The viability of the biological material at the time of        deposit will be tested; and    -   5. The deposit will be replaced if it should ever become        unavailable.

Access to this deposit will be available during the pendency of thisapplication to persons determined by the Commissioner of Patents andTrademarks to be entitled thereto under 37 C.F.R. § 1.14 and 35 U.S.C. §122. Upon allowance of any claims in this application, all restrictionson the availability to the public of the variety will be irrevocablyremoved by affording access to a deposit of at least 625 seeds of thesame seed source with ATCC. The Applicant does not waive anyinfringement of their rights granted under this patent or under thePlant Variety Protection Act (7 U.S.C. § 2321 et seq.). U.S. PlantVariety Protection of SO-110 is being applied for. Unauthorized seedmultiplication prohibited.

EXAMPLES

It should be understood that the examples and embodiments describedherein are for illustrative purposes only and that various modificationsor changes in light thereof will be suggested to persons skilled in theart and are to be included within the spirit and purview of thisapplication.

Example 1—Testing of Seed Quality Traits

The present invention is based on the development of true-bred camelinaseeds with unique characteristics, including, for example, prolongedseed filling period, increased seed oil content, decreased test weightand an increased seed weight. Advantageously, in some embodiments, theunique characteristics include, and/or contribute to, increased oil andgrain yields.

The oil content in the camelina seed was determined using contiguouswave low-resolution nuclear magnetic resonance (NMR) spectrometry at theSeed Lab at Oregon State University (Seed Science & Technology,Department of Crop and Soil Sciences, Oregon State University,Corvallis, Oreg., 97331).

Example 2—Agronomic Performance Field Evaluation of Camelina sativa (L.)SO-110

During the spring of 2010, 2011, and 2019, the performance of SO-110 wasevaluated in representative areas within the Pacific Northwest and theNorthern High Plains of the US including Oregon (Pendleton), Montana(Amsterdam, Bozeman, Huntley, Moccasin and Havre), Nebraska(Scottsbluff), Washington (Dusty), Wyoming (Lingle) and North Dakota(Carrington), USA. Two standard camelina varieties, “Calena” and “BlaineCreek” were included in these trials as controls for comparativepurposes. These evaluations were carried out under standard productionpractices.

Combined across all years and all environments, the average grain yieldfor SO-110 was 1,582 Lb/ac (range of 948 to 2,501 Lb/ac), which was11.6% higher than the mean yield of Calena (1,418 Lb/ac; range of 747 to2240 Lb/ac) and 16.9% higher than Blaine Creek (1,353 Lb/ac; range of683 to 2516 Lb/ac) (Table 1).

SO-110 has a linear stability coefficient (b) of 1.05 and a variancedeviation from linear regression (σ²) of 19700 (FIGS. 1-2 ). Thus,variety SO-110 should be considered a high-yielding variety with broadadaptability to high-input growing conditions.

Combined across all years and all environments, the average seed oilcontent for SO-110 was 36.2% (range of 29.8 to 43.5%), which was 1.7%higher than Calena (35.6%, range of 27.4 to 42%) and 0.3% higher thanBlaine creek (36.1%, range of 28.5 to 42.4%) (Table 2).

Combined across all years and all environments, the average oil yieldfor SO-110 was 573 Lb/ac (range of 303 to 981 Lb/ac), which was 11.6%higher than the mean yield of Calena (512 Lb/ac; range of 205 to 888Lb/ac) and 14.9% higher than Blaine Creek (499 Lb/ac; range of 195 to906 Lb/ac) (Table 3). Thus, SO-110 should be considered a higheroil-yielding variety.

In general, other than a relatively lower test weight (e.g., 50.0Lb/Bu), relatively longer seed filling period (e.g., 33 days) and anincreased seed weight (e.g., 1.38 g/1,000), SO-110 exhibited agronomicand phenologic characteristics that were comparable to the controlvarieties (Tables 4-8).

TABLE 1 Grain yield (Lb/ac) of camelina variety SO-110 and other controlcamelina varieties Bozeman Carrington Dusty Havre Huntley LinglePendleton Amsterdam Carrington Variety 2010 2010 2010 2010 2010 20102010 2011 2011 SO-110 1109 2409 1098 2501 1816 1177 1914 1643 1305Calena 954 2065  981 2228 1558 1484 1395 1374 1100 Blaine Creek 852 19681025 1678 1645 1350 1868 1202 872 Mean 972 2147 1034 2136 1673 1337 17251406 1092 p value ** NS NS NS NS NS * * ** CV (%) 6  12  16  14  11  1611 14 11 LSD (0.05) 134.7 — — — — — 419.8 346.6 214.0 Havre HuntleyPendleton Huntley Moccasin Pendleton Scottsbluff Variety 2011 2011 20112019 2019 2019 2019 Combined SO-110 1583 1167 1622 948 1563 2470 9951582 Calena 1457 1373 1701 776 1256 2240 747 1418 Blaine Creek 1112 12181717 714 1236 2516 683 1353 Mean 1384 1252 1680 812 1351 2409 808 1451 pvalue * NS NS NS NS NS ** *** CV (%) 14  34   9  22  13   7 7 14 LSD(0.05) 370.6 — — — — — 129.9 81.1 *, **, *** Significant at the 0.05,0.01, and 0.001 probability levels. NS Not significant.

TABLE 2 Seed oil content (%) of camelina variety SO-110and other controlcamelina varieties Bozeman Carrington Dusty Havre Huntley LinglePendleton Amsterdam Carrington Variety 2010 2010 2010 2010 2010 20102010 2011 2011 SO-110 41.0 39.6 35.6 39.5 40.2 33.5 36.5 35.5 29.8Calena 40.0 39.8 34.8 40.2 39.2 32.9 36.7 34.8 29.1 Blaine Creek 40.439.7 35.7 40.8 40.3 31.4 37.5 35.7 29.9 Mean 40.4 39.7 35.3 40.2 39.932.6 36.9 35.4 29.6 p value NS NS NS NS NS NS NS NS NS CV (%) 2  1  4 3  3  4  2  2  2  LSD (0.05) — — — — — — — — — Havre Huntley PendletonHuntley Moccasin Pendleton Scottsbluff Variety 2011 2011 2011 2019 20192019 2019 Combined SO-110 36.3 33.0 43.5 35.1 35.6 34.1 30.5 36.2 Calena35.5 33.0 42.0 35.1 35.2 33.8 27.4 35.6 Blaine Creek 37.3 33.2 42.4 35.233.9 35.5 28.5 36.1 Mean 36.4 33.1 42.6 35.1 34.9 34.5 28.8 36.0 p valueNS NS NS NS NS ** NS *** CV (%) 2  1  2  2  6  1 4  3 LSD (0.05) — — — —— 1.0 — 0.4 **, *** Significant at the 0.01, and 0.001 probabilitylevels. NS Not significant.

TABLE 3 Oil yield (lb/ac) of camelina variety SO-110 and other controlcamelina varieties Bozeman Carrington Dusty Havre Huntley LinglePendleton Amsterdam Carrington Variety 2010 2010 2010 2010 2010 20102010 2011 2011 SO-110 456 954 394 981 729 394 698 585 381 Calena 382 822339 888 614 489 510 479 320 Blaine Creek 345 781 365 683 664 426 697 430261 Mean 394 852 366 851 669 436 635 498 321 p value * NS NS * NS NS NSNS * CV (%) 7  12  17 11  12  18 12 16 14 LSD (0.05) 60.8 — — 211.3 — —— — 84.9 Havre Huntley Pendleton Huntley Moccasin Pendleton ScottsbluffVariety 2011 2011 2011 2019 2019 2019 2019 Combined SO-110 515 337 706333 556 843 303 573 Calena 509 453 715 274 443 764 205 513 Blaine Creek415 404 727 252 425 906 195 499 Mean 480 398 716 287 475 838 234 528 pvalue * NS NS NS NS NS ** *** CV (%) 6  37  8 22  16  9 11 15 LSD (0.05)80.1 — — — — — 55.8 31.0 *, **, *** Significant at the 0.05, 0.01, and0.001 probability levels. NS Not significant.

TABLE 4 Days to 50% flowering of camelina variety SO-110 and othercontrol camelina varieties Bozeman Carrington Dusty Havre Huntley LinglePendleton Amsterdam Carrington Variety 2010 2010 2010 2010 2010 20102010 2011 2011 SO-110 — 51 73 72 68 67 — 51 43 Calena — 50 73 72 69 67 —51 43 Blaine Creek — 50 72 70 68 66 — 49 43 Mean — 50 73 71 68 67 — 5043 p value — NS NS NS NS NS — *** * CV (%) —  1 1  3 1 1 — 1 1 LSD(0.05) — — — — — — — 0.6 0.6 Havre Huntley Pendleton Huntley MoccasinPendleton Scottsbluff Variety 2011 2011 2011 2019 2019 2019 2019Combined SO-110 63 85 42.5 55 60 56 — 62 Calena 62 85 43.3 56 64 58 — 63Blaine Creek 62 84 42.5 55 61 56 — 62 Mean 62 85 43.0 55 62 56 — 62 pvalue NS NS * NS *** *** — *** CV (%)  1  1 1  2 1 1 — 1 LSD (0.05) — —0.6 — 1.5 1.0 — 0.4 *, *** Significant at the 0.05 and 0.001 probabilitylevels. NS Not significant.

TABLE 5 Seed filling days of camelina variety SO-110 and other controlcamelina varieties Bozeman Carrington Dusty Havre Huntley LinglePendleton Amsterdam Carrington Variety 2010 2010 2010 2010 2010 20102010 2011 2011 SO-110 — 29 34 40 36 — — 34 29 Calena — 28 33 39 36 — —33 29 Blaine Creek — 28 35 36 36 — — 34 28 Mean — 28 34 38 36 — — 33 28p value — NS NS * NS — — NS NS CV (%) —  4  3 3 2 — —  2  4 LSD (0.05) —— — 2.4 — — — — — Havre Huntley Pendleton Huntley Moccasin PendletonScottsbluff Variety 2011 2011 2011 2019 2019 2019 2019 Combined SO-11033 29 — 34 27 34 — 33 Calena 33 28 — 34 25 33 — 32 Blaine Creek 33 27 —32 27 33 — 32 Mean 33 28 — 34 26 33 — 32 p value NS NS — NS * NS — ***CV (%)  4  4 — 7 4  4 — 4 LSD (0.05) — — — — 2.0 — — 0.5 *, ***Significant at the 0.05 and 0.001 probability levels. NS Notsignificant.

TABLE 6 Plant height (in) of camelina variety SO-110 and other controlcamelina varieties Bozeman Carrington Dusty Havre Huntley LinglePendleton Amsterdam Carrington Variety 2010 2010 2010 2010 2010 20102010 2011 2011 SO-110 — 31 31 33 40 29 — 26 33 Calena — 29 31 35 40 28 —26 33 Blaine Creek — 31 31 33 39 27 — 25 30 Mean — 30 31 34 40 28 — 2632 p value — NS NS NS NS NS — NS NS CV (%) —  8  2  6  3  5 —  3  8 LSD(0.05) — — — — — — — — — Havre Huntley Pendleton Huntley MoccasinPendleton Scottsbluff Variety 2011 2011 2011 2019 2019 2019 2019Combined SO-110 28 26 35 27 37 36 30 32 Calena 29 29 34 29 38 33 28 32Blaine Creek 27 29 36 29 35 36 29 31 Mean 28 28 35 28 37 35 29 31 pvalue NS NS NS NS NS NS NS NS CV (%)  6 11  8  8  6  7  4  6 LSD (0.05)— — — — — — — — NS Not significant.

TABLE 7 Seed weight (g/1,000) of camelina variety SO-110 and othercontrol camelina varieties Bozeman Carrington Dusty Havre Huntley LinglePendleton Amsterdam Carrington Variety 2010 2010 2010 2010 2010 20102010 2011 2011 SO-110 1.51 1.55 1.39 1.59 1.36 1.59 1.37 1.35 1.05Calena 1.36 1.41 1.34 1.48 1.33 1.55 1.41 1.17 0.95 Blaine Creek 1.361.43 1.33 1.55 1.21 1.45 1.37 1.12 0.90 Mean 1.41 1.46 1.35 1.54 1.301.53 1.38 1.21 0.97 p value NS *** NS NS * NS NS *** NS CV (%) 5 2 5 7  3 3   7   4 8   LSD (0.05) — 0.7 — — 0.1 — — 0.1 — Havre HuntleyPendleton Huntley Moccasin Pendleton Scottsbluff Variety 2011 2011 20112019 2019 2019 2019 Combined SO-110 1.35 1.18 1.26 — — — — 1.38 Calena1.11 1.04 1.18 — — — — 1.28 Blaine Creek 1.05 0.95 1.15 — — — — 1.24Mean 1.17 1.06 1.20 — — — — 1.30 p value ** NS * — — — — *** CV (%) 9 33 — — — — 5 LSD (0.05) 0.2 — 0.1 — — — — 0.03 *, **, *** Significant atthe 0.05, 0.01, and 0.001 probability levels. NS Not significant.

TABLE 8 Test weight (lb/Bu) of camelina variety SO-110 and other controlcamelina varieties Bozeman Carrington Dusty Havre Huntley LinglePendleton Amsterdam Carrington Variety 2010 2010 2010 2010 2010 20102010 2011 2011 SO-110 51.2 51.8 — 51.0 50.9 — — 50.4 47.0 Calena 52.952.9 — 52.4 52.5 — — 51.8 49.5 Blaine Creek 52.9 52.7 — 52.4 52.4 — —51.7 48.4 Mean 52.4 52.5 — 51.9 52.0 — — 51.3 48.3 p value * * — *** ***— — *** *** CV (%) 1 1 — <1 <1 — — 1 1 LSD (0.05) 1.1 0.7 — 0.3 0.2 — —0.5 0.9 Havre Huntley Pendleton Huntley Moccasin Pendleton ScottsbluffVariety 2011 2011 2011 2019 2019 2019 2019 Combined SO-110 51.1 49.050.7 46.0 49.4 51.1 — 50.0 Calena 52.0 50.5 52.3 46.7 50.9 52.1 — 51.4Blaine Creek 51.9 51.1 52.1 45.2 50.4 52.2 — 51.1 Mean 51.7 50.2 51.746.0 50.2 51.8 — 50.8 p value NS NS *** NS NS *** — *** CV (%) 1 3 <1 31 <1 — 1 LSD (0.05) — — 0.4 — — 0.3 — 0.3 *, *** Significant at the 0.05and 0.001 probability levels. NS Not significant.

Example 4—Development of SO-110

The present invention involves the development of true-bred camelinaseeds capable of growing and providing adequate seed yields underhigh-input, low-input, and/or dryland conditions in North America, andhaving but not being limited to the following characteristics comparedto other popular camelina varieties:

-   -   (i) a lower test weight of about, e.g., 50.0 Lb/Bu (range of        46.0 to 51.8 Lb/Bu);    -   (ii) a medium flowering period of about, e.g., 62 DAP (range of        42.5 to 85 DAP);    -   (iii) a medium seed filling period of about, e.g., 33 days        (range of 27 to 40 DAP);    -   (iv) a medium plant height of about, e.g., 32 inches, or 81.3 cm        (range of 26 to 40 inches (66 to 102 cm));    -   (v) a medium to high seed oil content of about, e.g., 36.2%        (range of 29.8 to 43.5%);    -   (vi) a high seed weight of about, e.g., 1.38 g/1,000 seeds        (range of 1.05 to 1.59);    -   (vii) a high oil yield of about, e.g., 573 lb/ac (range of 303        to 954 lb/ac); and    -   (viii) a high grain yield of about, e.g., 1,582 lb/ac (range of        948 to 2470 lb/ac).

Variety “SO-110” was developed from a cross between accession“Ames1043,” a material originated in Poland, and accession “PI 304268,”a material originated in Sweden. These accessions were evaluated foragronomic performance, adaptability, and oil quality attributes (fattyacid profile) across multiple locations during the period of 2006-2009(Tables 9 and 10).

TABLE 9 Specifics on field evaluations of parental accessions “Ames1043”and “PI 304268.” Set Entries Year Season Site(s) ^(†) 1 33 2006 Spring 82 45 2007 Spring 5 3 12 2007 Spring 11 4 20 2007/2008 Winter 1 5 20 2008Spring 25 6 20 2008 Spring 5 7 20 2008/2009 Winter 1 8 21 2009 Spring 79 20 2009 Spring 16 ^(†) Sites covered representative areas AZ, ID, MT,ND, NE, NM, OR, SD, WA, and WY in the USA, and in Alberta, Manitoba, andSaskatchewan, in Canada

TABLE 10 Agronomic performance of parental accessions Ames 1043 and PI304268. Accession Set 1 Set 2 Set 3 Set 4 Set 5 Set 6 Set 7 Set 8 Set 9Mean Grain yield (Lbs/ac) Ames 1043 1384.8 1710.3 1381 1736 1381 17961192 1744 1579 1545 PI 304264 1371.5 1574.2 1318 1676 — 1807 1705 — 15751256.5 1602.4 1330 1389 1197 1695 926 1669 1480 1394 Oil yield (Lbs/ac)Ames 1043 512 606 483 — 492 643 — 686 631 579 PI 304264 503 549 466 — —651 — 667 — 567 465 560 464 — 414 618 — 649 579 535 Oil content (%) Ames1043 37.50 36.2 34.97 — 34.25 36.40 — 39.14 39.65 36.88 PI 304264 37.0036.3 34.97 — — 36.03 — 38.66 — 36.59 36.90 35.75 34.89 — 33.84 36.21 —38.64 38.63 36.41 Seed weight (g/1000) Ames 1043 1.16 1.09 1.12 1.101.17 1.30 0.86 1.25 1.39 1.16 PI 304264 1.15 1.03 1.08 1.10 — 1.22 1.16— 1.12 1.04 0.99 1.10 0.95 1.08 1.19 0.74 1.15 1.33 1.06 Alpha-linolenicacid (%) Ames 1043 — 31.2 30.57 — — 33.99 — 35.28 — 32.75 PI 304264 —31.9 30.90 — — 34.99 — 36.33 — 33.53 — 32.0 31.41 — — 34.47 — 36.48 —33.59

A modified bulk-pedigree selection scheme was used for the developmentof SO-110 (Table 11). A cross between accession Ames1043 and accessionPI 304268 was made in a greenhouse in Bozeman, Mont. in the fall of2006. During the winter of 2006/2007, the seed of this hybrid wasadvanced to the F2 generation at a nursery in Yuma, Ariz., and in thespring of 2007, seed of this F2 population was grown in a selectionnursery near Bozeman, Mont. in duplicated plots (100 ft2 each).

At maturity, 20 individual plants were selected and harvested from thispopulation. Selection focused on early maturity, medium plant height,increased branch and pod number, and good overall appearance. A sampleof seed from each of these F3 families was collected and bulked, andduring the winter season of 2007/2008, this bulked seed was planted in awinter nursery near Yuma, Ariz. in duplicated plots (100 fWeach).

At maturity, an unrecorded number of individual plants were selected andharvested from this population using the criteria described above and arandom portion of seed from each plant was collected and bulked. Duringthe spring season of 2008 the bulked F4 seed from this population wasplanted in duplicated plots (100 ft² each) in a selection nursery inBozeman, Mont.

At maturity, 30 individual plants were selected from this populationusing the same criteria as before. Seed from each of these individual F5plants was planted in single rows in a nursery near Amsterdam, Mont. inthe spring of 2009.

At maturity, one line was selected based on the same selection criteriastated before. Seed of the selected line, C09-BZ-SB6_998_14 (SO-110),was purified and increased during the spring of 2010 in an isolatedstrip plot near Amsterdam, Mont.

TABLE 11 Breeding method used in development of SO-110. Gener- ationActivity Season Location F1 Crosses Fall Kalispell, 2006 MT F2 Seedadvance Winter Yuma, AZ 2006/ 2007 F3 Sample of seed from each F2 plantSpring Bozeman, bulked and planted in duplicated 2007 MT plots in aspring nursery. Single plant selections (20 individual plants selected).Selection criteria included early maturity, short plant stature,increased branching, increased pod number, good overall appearance. F4Sample of seed from each F3 line Winter Yuma, AZ bulked and planted in awinter 2007/ nursery. Single plant selections 2008 (unrecorded number ofindividual plants selected), selection criteria included early maturity,short stature, increased branching, increased pod number, overall goodappearance. F5 Sample of seed from each F4 line Spring Bozeman, bulkedand planted in duplicated 2008 MT plots in a spring nursery. Singleplant selections (30 individual plants selected), selection criteriaincluded early maturity, short stature, increased branching, increasedpod number, overall good appearance. F6 Sample of seed from selectedSpring Amsterdam, individual plants from the F5 2009 MT populationplanted in single-row plots in a nursery. Experimental lineC09-BZ-SB6_998_14 (SO-110) was selected. Selection criteria includedearly maturity, short plant stature, increased branching, increased podnumber, good overall appearance. F7 Seed increase and seed purificationSpring Amsterdam, in isolation field. 2010 MT F7 Multilocation yieldevaluation trials. Spring Bozeman, Seed oil quality evaluations. 2010MT; Carrington, ND; Dusty, WA, Havre, MT; Huntley, MT; Lingle, WYPendleton, OR Saskatoon, SK, CAN F8 Multilocation yield evaluationtrials. Spring Amsterdam, Seed oil quality evaluations. 2011 MTCarrington, ND; Havre, MT; Huntley, MT; Pendleton, OR F9 Multilocationyield evaluation trials. Spring Huntley, Seed oil quality evaluations.2019 MT; Moccasin, MT; Pendleton, OR; Scottsbluff, NE.

Example 5— Characteristics of Camelina Sativa (L.) Variety SO-110

Breeder seed of variety SO-110 and other plant materials, includingSO-50 (a patented variety used as a control variety to determine therelative value of SO-110 (see U.S. Pat. No. 8,319,021)), was grown in agrowth chamber in Great Falls, MT. Throughout the life cycle, the growthchamber was kept at an average temperature of 65° F. (range of 61° F. to72° F.), with a photoperiod of 16 h light and 8 h dark. Light intensitywas adjusted to 250 μmol/m²/s at bench height and to 350 μmol/m²/s atthe top of the canopy. Relative humidity was set to approximately 65%.

Seeds of these varieties were planted in single pots 6 inches indiameter and 5.75 inches in height at a rate of 2 seeds per pot in aSunshine Mix #4 grow soil media substrate. To accommodate for room forother plant materials, SO-110 was planted in 17 pots and SO-50 in 9pots. Once the seedlings reached the 3-leaf stage, they were thinned toonly one seedling per pot. Plants were fertilized with a 20-20-20 mix,providing an equivalent of 350 lb. of nitrogen per acre. Plants werewatered daily as needed by ensuring the soil was approximately at waterholding capacity. The data collected from these plants included plantheight, raceme number, main raceme length (cm), inflorescence height(cm), pod number, total pod weight (g), seeds per pod, seed number,1000-seed weight (g), and seed yield per plant (g). Plants wereharvested by hand once they reached the maturity stage.

Under controlled conditions, SO-110 grew taller than SO-50 (averageplant height of 81 cm vs. 77 cm). The inflorescence structure wascomparable between SO-110 and SO-50, although SO-110 had a slightlylonger inflorescence height (83 cm vs. 81 cm). Pod development wassignificantly enhanced in SO-110 compared to SO-50 (pod number 744 vs.695, total pod weight 14.1 g vs. 12.5 g), and the number of seeds perplant were significantly higher in SO-110 compared to SO-50 (7768 vs.7075). Seed yield per plant was higher in SO-110 compared to SO-50 (8.0g and 7.4 g, respectively). Thus, both number of seeds per plant andseed yield per plant constituted the main, unique attributes of varietySO-110.

TABLE 12 Yield component attributes of variety SO-110 Plant trait MeanMax Min SD SO-110 Plant Height (cm) 81 90 69 6.4 Raceme Number 17 22 142.2 Main Raceme Length (cm) 42 48 36 3.3 Inflorescence Height (cm) 83 9772 6.0 Pod Number 744 939 470 138.9 Total pod weight (g) 14.1 25.0 9.73.6 Seeds Per Pod 10 14 7 1.5 Seed Number 7768 9886 4703 1633.11000-Seed Weight (g) 1.05 1.30 0.84 0.13 Seed Yield Per Plant (g) 8.010.7 5.7 1.3 SO-50 Plant Height (cm) 77 83 70 5.5 Raceme Number 18 21 132.4 Main Raceme Length (cm) 40 51 34 5.3 Inflorescence Height (cm) 81 9070 5.2 Pod Number 695 897 518 108.2 Total pod weight (g) 12.5 16.8 9.02.3 Seeds Per Pod 10 12 8 1.1 Seed Number 7075 9892 4600 1656.91000-Seed Weight (g) 1.06 1.28 0.89 0.13 Seed Yield Per Plant (g) 7.49.2 4.8 1.4

All publications, patents, and patent publications cited areincorporated by reference herein in their entirety for all purposes.Also incorporated by reference herein are nucleic acid sequences andpolypeptide sequences deposited into the GenBank, which are cited inthis specification.

While the invention has been described in connection with specificembodiments thereof, it will be understood that it is capable of furthermodifications and this application is intended to cover any variations,uses, or adaptations of the invention following, in general, theprinciples of the invention and including such departures from thepresent disclosure as come within known or customary practice within theart to which the invention pertains and as may be applied to theessential features hereinbefore set forth and as follows in the scope ofthe appended claims.

REFERENCES

-   Anonymous. 2009. 14 Airlines sign landmark MOU for Camelina-based    renewable jet fuel & green diesel, Dec. 15, 2009. Business Wire.-   Berti, M. T. and A. A. Schneiter. 1993. Preliminary agronomic    evaluation of new crops for North Dakota. p. 105-109. In: J. Janick    and J. E. Simon (eds.), New crops. Wiley, New York.-   Bouby, L. 1998. Two early finds of gold-of-pleasure (Camelina sp.)    in middle Neolithic and Chacolithic sites in western France.    Antiquity, 72: 391-398.-   Brown et al. 1991. Tetrahedron, Vol. 47, Iss. 24, 3909-3914.-   Budin, J. T., W. M. Breene and D. H. Putnam, 1995. Some    compositional properties of Camelina (Camelina sativa (L.) Crantz)    seeds and oils. Journal of the American Oil Chemists Society, 72:    309-315.-   Gugel, R. K. and K. C. Falk. Agronomic and seed quality evaluation    of Camelina sativa in western Canada. 2006. Canadian Journal of    Plant Science, 86:1047-1058.-   Eberhart, S. A., and W. A. Russell, 1966. Stability parameters for    comparing varieties. Crop. Sci., 6: 36-40.-   Finlay, K. W., and G. N. Wilkinson, 1963. The analysis of adaptation    in a plant-breeding programme. Aust. J. Agr. Res., 14: 742-754.-   Schultze-Motel, J., 1979. Die Anbaugeschichte des Leindotters,    Camelina sativa (L.) Crantz.-   Pavlista and Baltensperger, 2007, Phenology of Oilseed Crops for    Bio-Diesel in the High Plains.-   Pilgeram et al., 2007, Camelina sativa, A Montana Omega-3 and Fuel    Crop, Issues in new crops and new use. InL J. Janick and A Whipkey    (eds.). ASHS Press, Alexandria, Va.-   Public Law 110-140. Energy Independence and Security Act of 2007.-   Putnam, D. H., J. T. Budin, L. A. Field, and W. M. Breene. 1993.    Camelina: A promising low-input oilseed. p. 314-322. In J. Janick,    and J. E. Simon (eds), New Crops, Exploration, Research and    Commercialization, John Wiley and Sons, Inc. New York, USA.-   Robinson, R. G. 1987. Camelina: a useful research crop and a    potential oilseed crop. University of Minnesota Agric. Exp. Stn.    Bull. 579-1987 (Item No. AD-SB-3275), pp. 1-12.-   Shonnard, D. R., L. Williams; and T. N. Kalnesc. 2010.    Camelina-Derived Jet Fuel and Diesel: Sustainable Advanced Biofuels.    Environmental Progress & Sustainable Energy, 29:382-392.-   Vollmann, J., A. Damboeck, A. Eckl, H. Schrems, and P.    Ruckenbauer. 1996. Improvement of Camelina sativa, an underexploited    oilseed. p. 357-362. In: J. Janick (ed.), Progress in new crops.    ASHS Press, Alexandria, Va.

We claim:
 1. A seed of Camelina sativa (L.) variety designated “SO-110,”wherein a representative sample of seed of said variety has beendeposited under ATCC Accession No. PTA-126939.
 2. A Camelina sativa (L.)plant, or a part thereof, produced by growing the seed of claim
 1. 3. ACamelina sativa (L.) plant, or a part thereof, having all thephysiological and morphological characteristics of Camelina sativa (L.)variety “SO-110,” wherein a representative sample of seed of saidvariety has been deposited under ATCC Accession No. PTA-126939.
 4. Atissue culture of regenerable cells produced from the plant or plantpart of claim
 2. 5. The tissue culture of claim 4, wherein said cells ofthe tissue culture are produced from a plant part selected from thegroup consisting of embryos, meristematic cells, leaves, pollen, root,root tips, stems, anther, pistils, pods, flowers and seeds.
 6. ACamelina sativa (L.) plant regenerated from the tissue culture of claim5, said plant having the morphological and physiological characteristicsof Camelina sativa (L.) variety “SO-110,” wherein a representativesample of seed bass has been deposited under ATCC Accession No.PTA-126939.
 7. A method for producing a Camelina seed comprisingcrossing a first parent Camelina plant with a second parent Camelinaplant and harvesting the resultant hybrid bean seed, wherein said firstparent Camelina plant or second parent Camelina plant is the Camelinasativa (L.) plant of claim
 2. 8. A hybrid Camelina seed produced by themethod of claim
 7. 9. A method for producing an herbicide-resistantCamelina plant comprising transforming the Camelina saliva (L.) plant ofclaim 2 with a transgene that confers herbicide resistance to anherbicide selected from the group consisting of imidazolinone,sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine, andbenzonitrile.
 10. An herbicide resistant Camelina plant, or a partthereof, produced by the method of claim
 9. 11. A method for producingan insect resistant Camelina plant comprising transforming the Camelinasaliva (L.) plant of claim 2 with a transgene that confers insectresistance.
 12. An insect resistant Camelina plant, or a part thereof,produced by the method of claim
 11. 13. A method for producing a diseaseresistant Camelina plant comprising transforming the Camelina sativa(L.) plant of claim 2 with a transgene that confers disease resistance.14. A disease resistant Camelina plant, or a part thereof, produced bythe method of claim
 13. 15. A method of introducing a desired trait intoCamelina sativa (L.) variety “SO-110” comprising: (a) crossing aCamelina saliva (L.) variety “SO-110” plant grown from Camelina saliva(L.) variety “SO-110” seed, wherein a representative sample of seed hasbeen deposited under ATCC Accession No. PTA-126939, with anotherCamelina plant that comprises a desired trait to produce F1 progenyplants; (b) selecting one or more progeny plants that have the desiredtrait to produce selected progeny plants; (c) crossing the selectedprogeny plants with the Camelina saliva (L.) variety “SO-110” plants toproduce backcross progeny plants; (d) selecting for backcross progenyplants that have the desired trait and physiological and morphologicalcharacteristics of Camelina saliva (L.) variety “SO-110” to produceselected backcross progeny plants; and (e) repeating steps (c) and (d)three or more times in succession to produce selected fourth or higherbackcross progeny plants that comprise the desired trait and thephysiological and morphological characteristics of Camelina saliva (L.)variety “SO-110.”
 16. A Camelina plant produced by the method of claim15, wherein the plant has the desired trait and otherwise all thephysiological and morphological characteristics of Camelina saliva (L.)variety “SO-110.”
 17. A method for producing Camelina saliva (L.)variety “SO-110” seed comprising crossing a first parent Camelina saliva(L.) plant with a second parent Camelina saliva (L.) plant andharvesting the resultant Camelina saliva (L.) seed, wherein both saidfirst and second Camelina saliva (L.) plants are the Camelina saliva(L.) plant of claim
 4. 18. The Camelina plant of claim 16, wherein thedesired trait is herbicide resistance and the resistance is conferred toan herbicide selected from the group consisting of imidazolinone,sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine, andbenzonitrile.
 19. The Camelina plant of claim 16, wherein the desiredtrait is insect resistance and the insect resistance is conferred by atransgene encoding a Bacillus thuringiensis endotoxin.
 20. The Camelinaplant of claim 16, wherein the desired trait is selected from the groupconsisting of insect resistance, disease resistance, water stresstolerance, heat tolerance, improved shelf life, and improved nutritionalquality.